
Vena8 Endothelial+ Biochip

The Vena8 Endothelial+™ biochip is ideal for cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow. Ideal for use with PCR. Compatible with brightfield, phase contrast imaging and fluorescence microscopy.
Features:
- Cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.
- Cells cultured within the biochip channels may be retrieved post-assay for subsequent studies (up to 320,000 cells); e.g. PCR. Substrate is removed from biochip and cells are scraped off.
- Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
- No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
- No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable. Dead volume is at input port ~ 0.1μL.
Applications: Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.
Specifications:
Substrate thickness | 0.5mm = 500μm |
Substrate material | High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy |
Number of channels per biochip | 8 |
Height of channels | 0.12mm = 120μm |
Width of channels | 0.8mm = 800μm |
Length of channels | 28mm = 2.8cm |
Volume of each channel | 2.69μL |
Volume of protein required for coating | 12μL (e.g. fibronectin to promote adhesion of cells for culture) |
Volume of cell sample required for seeding | 5μL; cell concentration = 15 x 106 cells/mL |
Growth area per channel | 0.096 mm2 |
Typical number of HUVECs cultured per channel | ~ 40,000 cells per channel |
Typical number of cells retrieved from one chip | ~ 320,000 cells per chip |
Volume of reservoir to hold sample | 0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed |