Vena8 with Glass Coverslip (Adhered / Not Adhered; Treated / Not Treated)
The Vena8™ biochip with/without glass coverslip adhered; with/without treatment for cell rolling and adhesion on proteins; seeding of cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow. Compatible with brightfield, phase contrast imaging, immunofluorescence and confocal microscopy.
- Cell rolling and adhesion on proteins; seeding of cells or microbes and subsequent study of drug interaction, imaging or molecular biology studies under shear flow.
- Biochips are supplied with or without glass coverslip attached.
- Glass coverslip may be treated or non-treated; e.g. glass may be treated with chelated Cu2+ ion tethered to the high-density PEG coating - surface of the microchannels may then be easily coated via pipetting and the protein binds via the poly-histidine tag.
- Biochips available for low or high flow rates / shear stresses.
- Ideal for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
- Perfect for high flow rates / high shear stresses e.g. platelet adhesion, aggregation and thrombi formation studies for thrombosis using whole blood. In standard flow chambers / perfusion chambers; significant volumes of blood would be required making the assay impossible.
- Suitable for biofilm applications; particularly Vena8 biochips supplied without glass coverslip: user may attach any type of coupon to bottom of the biochip.
- No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
- No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable.
Applications: Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); immunofluorescence and confocal microscopy.
|Substrate thickness||0.17mm = 170μm|
|Substrate material||Glass, compatible with immunofluorescence and confocal microscopy|
|Number of channels per biochip||8|
|LOW FLOW RATE BIOCHIPS:|
|Channel dimensions||160μm (H) x 1600μm (W) x 28mm ≡ 0.16mm (H) x 1.6mm (W) x 28mm|
|Volume of each channel
|Volume of protein required for coating||~20μL|
|HIGH FLOW RATE BIOCHIPS:|
||80μm (H) x 800μm (W) x 28mm ≡ 0.08mm (H) x 0.8mm (W) x 28mm|
|Volume of each channel
|Volume of protein required for coating||10μL|
|Volume of reservoir to hold sample||0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed|
Thrombosis assays - recommendations for whole blood:
Stem cell assays - recommendations for stem cell culture:
Biofilm assays - recommendations for biofilm culture: