The VenaT4™ biochip for chemotaxis, transmigration and invasion assays. Compatible with brightfield, phase contrast imaging and fluorescence microscopy.
- Chemotaxis, transmigration and invasion assays: in-vivo like setting; cells adhere from flow and transmigrate through embedded microporous membrane (2-10 μm pore sizes) into underlying microwell containing chemoattractant.
- Suitable for ECM-matrix, collagen gels, hydrogels, matrigel or similar aqueous gels.
- Cells may also be cultured in the underlying microwell with/without gel.
- Faster than traditional chemotaxis assays due to reduced distance in microchannel format.
- Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
- No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
- No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable. Dead volume is at input port ~ 0.1μL.
Applications: Chemotaxis, transmigration and invasion assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.
|Substrate thickness||0.5mm = 500μm|
|Substrate material||High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy|
|Number of channels / microwells per biochip||4|
|Height of channels||0.1mm = 100μm|
|Width of channels||0.8mm = 800μm|
|Length of channels||28mm = 2.8cm|
|Volume of each channel||2.253μL|
|Volume of protein required for coating channel||50μL|
|Volume of gel required for microwell||30μL|
|Volume of reservoir to hold sample||0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being perfor|