Vena8 Endothelial+ Biochip
The Vena8 Endothelial+™ biochip is ideal for cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow. Ideal for use with PCR. Compatible with brightfield, phase contrast imaging and fluorescence microscopy.
- Cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.
- Cells cultured within the biochip channels may be retrieved post-assay for subsequent studies (up to 320,000 cells); e.g. PCR. Substrate is removed from biochip and cells are scraped off.
- Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
- No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
- No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable. Dead volume is at input port ~ 0.1μL.
Applications: Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.
|Substrate thickness||0.5mm = 500μm|
|Substrate material||High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy|
|Number of channels per biochip||8|
|Height of channels||0.12mm = 120μm|
|Width of channels||0.8mm = 800μm|
|Length of channels||28mm = 2.8cm|
|Volume of each channel||2.69μL|
|Volume of protein required for coating||12μL (e.g. fibronectin to promote adhesion of cells for culture)|
|Volume of cell sample required for seeding||5μL; cell concentration = 15 x 106 cells/mL|
|Growth area per channel||0.096 mm2|
|Typical number of HUVECs cultured per channel||~ 40,000 cells per channel|
|Typical number of cells retrieved from one chip||~ 320,000 cells per chip|
|Volume of reservoir to hold sample||0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed|