
VenaT4 Biochip

The VenaT4™ biochip for chemotaxis, transmigration and invasion assays. Compatible with brightfield, phase contrast imaging and fluorescence microscopy.
Features:
- Chemotaxis, transmigration and invasion assays: in-vivo like setting; cells adhere from flow and transmigrate through embedded microporous membrane (2-10 μm pore sizes) into underlying microwell containing chemoattractant.
- Suitable for ECM-matrix, collagen gels, hydrogels, matrigel or similar aqueous gels.
- Cells may also be cultured in the underlying microwell with/without gel.
- Faster than traditional chemotaxis assays due to reduced distance in microchannel format.
- Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
- No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
- No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable. Dead volume is at input port ~ 0.1μL.
Applications: Chemotaxis, transmigration and invasion assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.
Specifications:
Substrate thickness | 0.5mm = 500μm |
Substrate material | High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy |
Number of channels / microwells per biochip | 4 |
Height of channels | 0.1mm = 100μm |
Width of channels | 0.8mm = 800μm |
Length of channels | 28mm = 2.8cm |
Volume of each channel | 2.253μL |
Volume of protein required for coating channel | 50μL |
Volume of gel required for microwell | 30μL |
Volume of reservoir to hold sample | 0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being perfor |