megantech
Polish Flag

Vena8 Endothelial+ Biochip



Vena8 Endothelial+

The Vena8 Endothelial+™ biochip is ideal for cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow. Ideal for use with PCR. Compatible with brightfield, phase contrast imaging and fluorescence microscopy.

Features:

  • Cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow. 
  • Cells cultured within the biochip channels may be retrieved post-assay for subsequent studies (up to 320,000 cells); e.g. PCR. Substrate is removed from biochip and cells are scraped off.
  • Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
  • No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
  • No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable. Dead volume is at input port ~ 0.1μL.

Applications: Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.


Specifications:

Substrate thickness  0.5mm = 500μm
Substrate material  High quality plastic, compatible with brightfield, phase contrast imaging and fluorescence microscopy
Number of channels per biochip  8 
Height of channels  0.12mm = 120μm
Width of channels  0.8mm = 800μm
Length of channels  28mm = 2.8cm
Volume of each channel  2.69μL
Volume of protein required for coating  12μL (e.g. fibronectin to promote adhesion of cells for culture)
Volume of cell sample required for seeding  5μL; cell concentration = 15 x 10cells/mL
Growth area per channel  0.096 mm2
Typical number of HUVECs cultured per channel   ~ 40,000 cells per channel
Typical number of cells retrieved from one chip  ~ 320,000 cells per chip 
Volume of reservoir to hold sample  0.5mL; also possible to connect via tubing to eppendorf if greater sample volume or high shear stress / flow rate assay is being performed



megantech

Vistitors: