RECIRCULATING PUMPS
Kima
Recirculating pump for Cell Culture in Flow Chambers
Recirculating pump for Cell Culture in Flow Chambers
CELL CULTURE UNDER FLOW: simple culture of endothelial cell monolayers under flow in flow chambers and 8 cell monolayers in Cellix's Vena8 Endothelial+ biochips within 24-48 hours. Culture can continue up to weeks if required.
FLOW RATES: ~250 - 600µL/min. If using Kima pump with Vena8 biochip range, the flow splitter will result in flow rates of ~30 - 75µL/min in each of the 8 channels of the biochip.
RECIRCULATING CULTURE MEDIA: saves reagents and no additional fluidic valves required.
INCUBATOR SAFE: Fits inside all standard CO incubators. No additional temperature or humidity control required.
PULSATILE: apply continuous or periodic pulses of culture media or reagent to cell monolayers in culture.
PROGRAMMABLE with KimaFlo software via your PC.
EASY TO USE: control up to 4 pumps with the same controller via KimaFlo software.
HOW DOES IT WORK?
Kima Recirculating Pump with Kima Controller
APPLICATIONS
Cell culture of endothelial cell monolayers for shear flow experiments.
Cell-cell adhesion shear flow assays on endothelial cell monolayers with Cellix's Mirus Evo Nanopump or ExiGo pump for studies such as:
Cardiovascular / Atherosclerosis: primary monocyte adhesion to coronary artery endothelial cells.
Inflammation: THP-1 monocyte adhesion to HUVECs under flow.
Asthma / COPD: eosinophil adhesion studies to human lung microvascular endothelial cells (HMVEC-L).
Oncology - Breast Cancer: MDA cells adhering to TNF-alpha stimulated HUVECs under shear flow.
Downloads
Tech Notes & Protocol
Quick Start Guides